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שלום,קיימת קורילציה ישירה בין רמות הגונדוטרופינים ופגיעה במבנה הגנטי של הביציות בזמן החלוקה המיוטית שלהן-חהלן המאמר המראה זאת בזמן הבשלת ביציות בתנאי המעבדה High follicle-stimulating hormone increases aneuploidy in human oocytes matured in vitro Yan-Wen Xu, M.D., Ph.D., Yue-Ting Peng, M.D., Bin Wang, M.D., Yan-Hong Zeng, B.Sc., Guang-Lun Zhuang, M.D., and Can-Quan Zhou, M.D. Reproductive Medical Center, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou, People’s Republic of China Objective: To study the effect of FSH on the aneuploidy risk of human oocytes matured in vitro. Design: Prospective study. Setting: Hospital-based IVF center. Patient(s): Patients with male factor infertility undergoing intracytoplasmic sperm injection (ICSI) cycles. Intervention(s): Immature oocytes were put into five groups according to the FSH concentration (0, 5.5, 22, 100, and 2,000 ng/mL) in in vitro maturation (IVM) medium. Spindles were observed under a polarized microscope before polar body biopsy. Fixed polar bodies and corresponding oocytes were examined on chromosomes 13, 16, 18, 21, and 22 by fluorescence in situ hybridization. Oocytes matured in 5.5 and 2,000 ng/mL FSH were immunostained for tubulin and chromatin. Main Outcome Measure(s): Aneuploidy rate, spindle visualization rate, and spindle morphology. Result(s): The frequency rates of aneuploidy were 26.7%, 23.3%, 36.75%, 46.67%, and 63.3% in the five FSH groups, respectively. There was a significantly higher aneuploidy rate in oocytes matured in the 2,000 ng/mL FSH group. The spindle visualization rates assessed under PolScope were not significantly different between aneuploid and normal oocytes. There was no difference in spindle morphology between the 2,000 and 5.5 ng/mL FSH groups. Conclusion(s): High-concentration FSH in IVM medium significantly increased the first meiotic division error, resulting in more aneuploid oocytes during IVM. (Fertil Steril 2011;95:99–104. 2011 by American Society for Reproductive Medicine.) Key Words: FSH, oocytes, in vitro maturation, aneuploidy מאמר נוסף מצביע על האפקט השליל על רירית הרחם Detrimental Effects of High-Dose Gonadotropin on Outcome of IVF Making a Case for Gentle Ovarian Stimulation Strategies  Authors Abstract Objective: Limited data identify detrimental influences of aggressive use of gonadotropins (G) for controlled ovarian hyperstimulation (COH); the underlying mechanisms however remain unclear. We report on the relationship between G dose and in vitro fertilization (IVF) cycle parameters (ovarian response, endometrial thickness [EMT]) and cycle outcome (implantation rate [IR] and clinical pregnancy [CP] rate) in a cohort of women undergoing IVF. Methods: Retrospective analysis of fresh embryo transfer (ET) cycles. Univariate and multivariable regression analyses assessed relationship between G dose and outcomes of interest. Results: Higher G dose related positively with advancing age (P < .001) and inversely with EMT (P < .001). The overall CP rate was 30%. Significantly lower IR (.003) and CP rate (.002) were observed across increasing tertiles of G dose. Increasing G dose was identified as an independent negative predictor of EMT after adjusting for age, COH protocol and duration, infertility diagnosis, and ovarian response (P = .016). Adjusting for age, suppression protocol (gonadotropin-releasing hormone agonist vs antagonist), infertility diagnoses, EMT, quality, and cleavage of ET, lower G dose was an independent positive predictor of CP rate (odds ratio for CP rate was 1.57 for G dose in middle compared to the highest G dose quartile (95% confidence interval 1.09-2.24). Stratified analyses identified detrimental associations of higher G dose with CP rate to be relevant in women aged 35 years and younger. Conclusions: Our analyses suggest detrimental influences of higher G dose on the endometrium and confirm the previously reported adverse association between higher G dose and IVF outcome. Gentler COH regimens may be of particular benefit in women aged ≤35 years.

שלום,קיימת קורילציה ישירה בין רמות הגונדוטרופינים ופגיעה במבנה הגנטי של הביציות בזמן החלוקה המיוטית שלהן-חהלן המאמר המראה זאת בזמן הבשלת ביציות בתנאי המעבדה High follicle-stimulating hormone increases aneuploidy in human oocytes matured in vitro Yan-Wen Xu, M.D., Ph.D., Yue-Ting Peng, M.D., Bin Wang, M.D., Yan-Hong Zeng, B.Sc., Guang-Lun Zhuang, M.D., and Can-Quan Zhou, M.D. Reproductive Medical Center, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou, People’s Republic of China Objective: To study the effect of FSH on the aneuploidy risk of human oocytes matured in vitro. Design: Prospective study. Setting: Hospital-based IVF center. Patient(s): Patients with male factor infertility undergoing intracytoplasmic sperm injection (ICSI) cycles. Intervention(s): Immature oocytes were put into five groups according to the FSH concentration (0, 5.5, 22, 100, and 2,000 ng/mL) in in vitro maturation (IVM) medium. Spindles were observed under a polarized microscope before polar body biopsy. Fixed polar bodies and corresponding oocytes were examined on chromosomes 13, 16, 18, 21, and 22 by fluorescence in situ hybridization. Oocytes matured in 5.5 and 2,000 ng/mL FSH were immunostained for tubulin and chromatin. Main Outcome Measure(s): Aneuploidy rate, spindle visualization rate, and spindle morphology. Result(s): The frequency rates of aneuploidy were 26.7%, 23.3%, 36.75%, 46.67%, and 63.3% in the five FSH groups, respectively. There was a significantly higher aneuploidy rate in oocytes matured in the 2,000 ng/mL FSH group. The spindle visualization rates assessed under PolScope were not significantly different between aneuploid and normal oocytes. There was no difference in spindle morphology between the 2,000 and 5.5 ng/mL FSH groups. Conclusion(s): High-concentration FSH in IVM medium significantly increased the first meiotic division error, resulting in more aneuploid oocytes during IVM. (Fertil Steril 2011;95:99–104. 2011 by American Society for Reproductive Medicine.) Key Words: FSH, oocytes, in vitro maturation, aneuploidy מאמר נוסף מצביע על האפקט השליל על רירית הרחם Detrimental Effects of High-Dose Gonadotropin on Outcome of IVF Making a Case for Gentle Ovarian Stimulation Strategies  Authors Abstract Objective: Limited data identify detrimental influences of aggressive use of gonadotropins (G) for controlled ovarian hyperstimulation (COH); the underlying mechanisms however remain unclear. We report on the relationship between G dose and in vitro fertilization (IVF) cycle parameters (ovarian response, endometrial thickness [EMT]) and cycle outcome (implantation rate [IR] and clinical pregnancy [CP] rate) in a cohort of women undergoing IVF. Methods: Retrospective analysis of fresh embryo transfer (ET) cycles. Univariate and multivariable regression analyses assessed relationship between G dose and outcomes of interest. Results: Higher G dose related positively with advancing age (P < .001) and inversely with EMT (P < .001). The overall CP rate was 30%. Significantly lower IR (.003) and CP rate (.002) were observed across increasing tertiles of G dose. Increasing G dose was identified as an independent negative predictor of EMT after adjusting for age, COH protocol and duration, infertility diagnosis, and ovarian response (P = .016). Adjusting for age, suppression protocol (gonadotropin-releasing hormone agonist vs antagonist), infertility diagnoses, EMT, quality, and cleavage of ET, lower G dose was an independent positive predictor of CP rate (odds ratio for CP rate was 1.57 for G dose in middle compared to the highest G dose quartile (95% confidence interval 1.09-2.24). Stratified analyses identified detrimental associations of higher G dose with CP rate to be relevant in women aged 35 years and younger. Conclusions: Our analyses suggest detrimental influences of higher G dose on the endometrium and confirm the previously reported adverse association between higher G dose and IVF outcome. Gentler COH regimens may be of particular benefit in women aged ≤35 years.

האם תוכל להסביר מתי עודף הורמוניים טוקסיים לאיכות הבצית ובאיזה מאפיינים האיכות נפגעת?

ובאיזה מאפיינים של איכות הביציות מינון עודף של הורמונים עלול לפגוע?

שלום,התגובה שלך לטיפול הורמונלי מסיבי אינה עומדת בציפיות ביחס לרמת ה-FSH שלך,וגילך,אולם קיימים מדדים נוספים להערכת הרזרבה השחלתית שלך כמו -AMH,antral follicle count(AFC,בכל מקרה ניתן בנסיבות אלו לבצע טיפול בפרוטוקול רך natural cycle,או modified natural cycle ולא להעמיס עודף הורמונים שלעיתים הינם טוקסיים לאיכות הביציות,ניתן להעזר בתוסף מזון DHEA